Cleaning Olive Oil Samples with Gel Permeation/Size Exclusion Chromatography

At Knauer, we believe that ‘Science Together’, is not just a catchy slogan but an ethos which is applied to all the research we enthusiastically undertake. Gel permeation chromatography (GPC) also called size exclusion chromatography (SEC) is one of many state-of-the-art extraction and identification techniques that our technicians specialise in. GPC/SEC is the most suitable sample preparation method in the cleaning of sample matrices like olive oil, as well as sample matrices of similar chemical character and consistency.

Olive Oil Pesticide Extraction through GPC/SEC

Utilizing the size exclusion chromatography solid phase material BioBeads SX-3 and Cyclohexane/ethyl acetate as a solvent, a comprehensive GPC purification procedure can be conducted using our proprietary ‘KNAUER AZURA GPC Cleanup System’. The system is ideal for removing undesirable molecules that might otherwise disturb analyses on other systems. Ours has a proven success rate of approximately 70%.

Particular Applications Ideal of Size Exclusion Chromatography

Size exclusion chromatography is especially suitable for applications that call for the separation of smaller, unidentifiable pesticides that are concealed within matrices of higher molecular weights, such as olive oil. The multi-loop testing process with a wash loop at the end, which our particular KNAUER AZURA GPC Cleanup System adheres to, drastically increases the method’s safety against cross-contamination.

KNAUER Standards and Services

In the area of size exclusion chromatography, our researchers at Knauer possess a definitive selection of customizable and adaptable testing and analysis methods to arrive at the desired results.


Directly applicable to the extraction of small amounts of potentially harmful pesticides contained within a solution, such as oil, the KNAUER AZURA system offers a great solution for the pre-treatment of such difficult matrices for the optimization of the subsequent analysis.

US EPA Method 3640A Calibration Standard

Known as a no-nonsense method of expedient pesticide extraction, samples that have undergone the US EPA method show much lower signs of contamination across the board.

High-Performance Liquid Chromatography (HPLC)

An extremely versatile separation technique, HPLC defines each internal component based on its interactions with the core of the sample, as the various elements are mixed together.

Read complete App Note: GPC Clean-up of Olive Oil Samples

For the chance to hear some of our industry experts explain the possible ways size exclusion chromatography could benefit you and your business, contact a member of our team today.

More HPLC food analysis 

Follow us