At Knauer, we believe that ‘Science Together’, is not just a catchy slogan but an ethos which is applied to all the research we enthusiastically undertake. Gel permeation chromatography (GPC) also called size exclusion chromatography (SEC) is one of many state-of-the-art extraction and identification techniques that our technicians specialise in. GPC/SEC is the most suitable sample preparation method in the cleaning of sample matrices like olive oil, as well as sample matrices of similar chemical character and consistency.
Olive Oil Pesticide Extraction through GPC/SEC
Utilizing the size exclusion chromatography solid phase material BioBeads SX-3 and Cyclohexane/ethyl acetate as a solvent, a comprehensive GPC purification procedure can be conducted using our proprietary ‘KNAUER AZURA GPC Cleanup System’. The system is ideal for removing undesirable molecules that might otherwise disturb analyses on other systems. Ours has a proven success rate of approximately 70%.
Particular Applications Ideal of Size Exclusion Chromatography
Size exclusion chromatography is especially suitable for applications that call for the separation of smaller, unidentifiable pesticides that are concealed within matrices of higher molecular weights, such as olive oil. The multi-loop testing process with a wash loop at the end, which our particular KNAUER AZURA GPC Cleanup System adheres to, drastically increases the method’s safety against cross-contamination.
KNAUER Standards and Services
In the area of size exclusion chromatography, our researchers at Knauer possess a definitive selection of customizable and adaptable testing and analysis methods to arrive at the desired results.
KNAUER AZURA GPC Cleanup System
Directly applicable to the extraction of small amounts of potentially harmful pesticides contained within a solution, such as oil, the KNAUER AZURA system offers a great solution for the pre-treatment of such difficult matrices for the optimization of the subsequent analysis.
US EPA Method 3640A Calibration Standard
Known as a no-nonsense method of expedient pesticide extraction, samples that have undergone the US EPA method show much lower signs of contamination across the board.
High-Performance Liquid Chromatography (HPLC)
An extremely versatile separation technique, HPLC defines each internal component based on its interactions with the core of the sample, as the various elements are mixed together.
Read complete App Note: GPC Clean-up of Olive Oil Samples
For the chance to hear some of our industry experts explain the possible ways size exclusion chromatography could benefit you and your business, contact a member of our team today.
Food analysis is essential to ensure product quality, execute regulations, and to comply with food standards, specifications, and labeling requirements.
HPLC analysis of pesticides determines the quality of food and increases the safety of the consumer. Especially in industrial agriculture a variety of pesticides are used, which can be detected by HPLC.
Food contaminant analysis of aflatoxins and other mycotoxins is very important, as aflatoxin and mycotoxin belong to one of the most serious groups of natural food contaminants. Find out how HPLC can support.