Two step purification is a special multicolumn chromatography solution. Two independent methods, each with their associated specific column, are used to realize the purification of the target molecule without manual interference. The principle here is that the protein sample is applied on the first column. During elution of the protein, the protein peak is detected triggering the collection of the eluted protein in a storage loop or storage vessel/container. The protein is then automatically applied on the second column to further enhance the quality and or purity of the purified protein. Several system set ups can be used to automate the purification. In the third section of the three-part guide a two step purification with the sample pump set up is discussed.
Flow scheme for the sample pump set up for two step purification
| Methode | FPLC |
|---|---|
| Modus | IEC |
| Schlüsselwörter | Two step purification, purification, protein purification, biochromatography, guide, sample pump set up |
| Version | Version 1 / 01/2021 |
VU 4.1 Ventilantrieb für V 4.1 Ventile
Artikelnummer: AWA01XA
Zum Produkt
Assistentenmodul VU 4.1 für Ventile V 4.1
Artikelnummer: EWA04
Zum Produkt
Multipositions Ventil, 8 Port, PEEK, PEEK, 1/16'', 240 bar, 0.75 mm Bohrgröße
Artikelnummer: AVS34CE
Zum Produkt
Ventil für die Auswahl von 5 Säulen, Flussumkehr und Bypass
Artikelnummer: AVZ52CE
Zum Produkt
Filterpatrone für Pumpe P 6.1L, Titan-Fritte, 2 µm Porengrösse. 50 ml/min maximaler Durchfluss, Filter mit hoher Kapazität, 60 µl Volumen, 3 Stk.
Artikelnummer: A9661
Zum Produkt
Inline Filter zum Schutz der Säule, mit 10 µm Porengröße
Artikelnummer: A3379
Zum Produkt
für bis zu 50 ml/min
Artikelnummer: SYS875413122
Zum ProduktWir helfen Ihnen gerne!