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Reduce pulsation – improve performance and increase longevity of your column using the pulse damper with the AZURA® SEC FPLC

Applikationsnr.: VTN0019

Reduce pulsation – improve performance and increase longevity of your column using the pulse damper with the AZURA® SEC FPLC

Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with the pressure sensitive columns used in size exclusion chromatography applications. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the compact pump AZURA P 4.1S. The pulse damper was able to reduce pressure pulsation by up to 92%. The best working conditions for the pulse damper were determined at small flow rates in combination with a low back pressure within the system. Reducing the degree of pressure pulsation prevents potential damage to the column packing and therefore it is highly recommended to include the pulse damper within the flow path of the AZURA SEC FPLC system. Here, two system configurations are reviewed in detail.

Technische Daten

Spezifikationen

Methode FPLC
Modus SEC
Substanzen -
Schlüsselwörter FPLC, SEC, pulse damper, AZURA SEC FPLC, biocompatible
CAS Nummer -
Version Version 1 / 10/2021

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