Extraction of natural components from plants that are used for medical treatments is widely applied. Often the extraction process results in an oil, such as the chamomile blue oil with many different compounds. Usually only some of these components are responsible for the medical properties of the whole extract. In chamomile oil, chamazulene is one of these medical active compounds. Here, the preparative purification of chamazulene by HPLC from chamomile blue oil is described.
VPH0071 Chromatogram chamazulene purification. Collected fraction highlighted in grey. 2 mL injection volume; x – chamazulene peak; C18 20 x 250 mm; 10 μm; 25 mL/min.
VPH0071 Overlay fraction from Fig. 4 in blue and chamazulene standard (0.75 mg/mL) in red on analytical column
| Methode | Preparative HPLC |
|---|---|
| Modus | RP |
| Substanzen | Chamazulene |
| Schlüsselwörter | Chamazulene, scale-up, preparative LC, RP, pharma, purification |
| CAS Nummer | 529-05-5 |
Purification of chamazulene by preparative HPLC and its scale-up
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