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Group separation with Sepapure Desalting on AZURA® Bio purification system

Applikationsnr.: VBS0075

Group separation with Sepapure Desalting on AZURA® Bio purification system

Size exclusion chromatography (SEC) is a popular FPLC techniques used for protein purification. Molecules are separated according to their size. Depending on the aim of the purification high resolution fractionation or group separation is used. This application describes group separation with Sepapure Desalting and shows examples for the separation of proteins from dyes and for protein desalting.

Technische Daten

Applikation

Methode FPLC

Trennbedingungen

Modus SEC
Substanzen Bovine Serum Albumin (BSA), 5-Carboxyfluorescein (5-FAM)
Schlüsselwörter SEC, Desalting, Buffer exchange, group seperation, FPLC, Sepapure Desalting, AZURA FPLC
CAS Nummer 9048-46-8, 76823-03-5

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