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Easy and fast isolation of rosmarinic acid from lemon balm with mass-directed purification

Applikationsnr.: VPH0067

Easy and fast isolation of rosmarinic acid from lemon balm with mass-directed purification

Rosmarinic acid is a natural product widely spread over different plant families. Preparative reversed-phase chromatography was used for the mass-directed purification of rosmarinic acid from a lemon balm extract. The AZURA® Prep System together with the 4000 MiD mass spectrometer was showed to be well suited for this application. 

  • Analytical chromatogram of the crude lemon balm extract at 280 nm; gradient separation 20 %-100 % acetonitrile, *rosmarinic acid peak

  • SIM (single ion monitoring) chromatogram of a purification run for the target mass of m/z 359.2

  • UV-Chromatogram of a purification run for the crude lemon balm extract at 280 nm, *rosmarinic acid peak

  • Analytical chromatogram of the fraction containing rosmarinic acid at 280 nm, isocratic separation 50/50 water/acetonitrile

Technische Daten

Spezifikationen

Methode LC-MS
Modus RP
Substanzen Rosmarinic Acid, lemon balm extract
Schlüsselwörter mass-directed purification, rosmarinic acid, AZURA® analytical system, AZURA® prep. system, 4000 MiD mass spectrometer
CAS Nummer 20283-92-5

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