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Determination of Amino acids with OPA

Applikationsnr.: VPH0010J

Determination of Amino acids with OPA

Amino acid analysis is a considerable application applied in research, clinical facilities and industrial processes. The application described in this study uses o-Phtalaldehyde (OPA) as pre-column derivatizing reagent. OPA is a well-known and reliable derivatization reagent for amino acid analysis. Its significantly lower price compared to other derivatization reagents like 6- aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) makes this reagent very attractive for the use in routine analyses.

Technische Daten

Applikation

Methode HPLC

Trennbedingungen

Modus RP
Substanzen Aspartic acid, Glutamic acid, Serine, Glutamine, Histidine, Glycine, Threonine, Arginine, Alanine, Tyrosine, Methionine, Valine, Phenylalanine, Isoleucine, Leucine, Lysine
Schlüsselwörter Amino acids, Precolumn derivatization, OPA, protein
CAS Nummer 56-84-8, 56-86-0, 56-45-1, 56-85-9, 71-00-1, 56-40-6, 72-19-5, 56-41-7, 60-18-4, 63-68-3, 72-18-4, 63-91-2, 73-23-5, 61-90-5, 56-87-1

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