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Performance Verification Standards für HPLC

Wann benutze ich welchen PV Standard?
 

Back-pressure range Type of detection Flow cell path length Injection: Probenschleifen Volumen PV Dokument Artikelnummer des PV standard Arttikelnummer der HPLC Säule
    mm µl      
UHPLC systems
(max. 1000 bar)
UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 10BE181E2F
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-2 10BE181E2F
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-1 10BE181E2F
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-2 10BE181E2F
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-3 10BE181E2F
HPLC Plus systems 
(max. 700 bar)
UV, DAD 10 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-4 15WE181E2J
UV, DAD 10 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 1 - 20 VPV-001: Analytical HPLC, UV detection A01260-3 15WE181E2J
UV, DAD 50 21 - 100 VPV-001: Analytical HPLC, UV detection A01260-2 15WE181E2J
FLD all 1 - 20 VPV-004: Analytical HPLC, FL detection A01262-1 15WE181E2J
FLD all 21 - 100 VPV-004: Analytical HPLC, FL detection A01262-2 15WE181E2J
HPLC and UHPLC systems RID all all VPV-002: Analytical HPLC, RI detection A01261-1 05WE184E2J
Preparative  HPLC systems UV, DAD < 2 all VPV-007: Preparative HPLC, UV detection A01264-1 05JE181E2J
  UV, DAD > 2 all VPV-007: Preparative HPLC, UV detection A01264-2 05JE181E2J
  RID all all VPV-008: Preparative HPLC, RI detection A01265-1 05IE184E2J

Definition of PV Standards:

Article number butyl benzoate 136-60-7 pentyl benzoate 2049-96-9 uridin58-96-8 deoxyuridin 951-78-0 anthracene 120-12-7 Solvent Volume
  mg/mL mg/mL mg/mL mg/mL µg/mL   ml
A01260-4 0,50 0,50       ACN/H2O 8:2 1,2
A01260-3 0,10 0,10       ACN/H2O 8:2 1,2
A01260-2 0,02 0,02       ACN/H2O 8:2 1,2
A01260-1 0,01 0,01       ACN/H2O 8:2 1,2
A01261-1     1,00 1,00   H2O 1,2
A01261-2     0,10 0,10   H2O 1,2
A01262-1         0,030 ACN/H2O 8:2 1,2
A01262-2         0,006 ACN/H2O 8:2 1,2
A01262-3         0,003 ACN/H2O 8:2 1,2
A01264-1   2,00       ACN/H2O 75:25 10
A01264-2   0,5       ACN/H2O 75:25 10
A01265-1     40     H2O 10

System Operation Qualification (OQ) Procedures for HPLC

The Operation Qualification includes extensive functionality tests of pump, autosampler and detector to prove that the system runs reliably within the documented specifications.

This table gives an overview of the available standards and equipment for a system OQ.
 

Article number Description Items included
A66310 Equipment for System OQ System OQ test document VOQ-SYS-01 Restriction capillary System OQ Kit of Caffeine Standards: Set 1
A66320 Equipment for System OQ System OQ Kit of Caffeine Standards: Set 2
A01269-10 System OQ Test Standard Set 1     System OQ Kit of Caffeine Standards: Set 1
A01269-20 System OQ Test Standard Set 2     System OQ Kit of Caffeine Standards: Set 2

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