Pressure fluctuations caused by pump pulsation can present a challenge in FPLC, especially when working with the pressure sensitive columns used in size exclusion chromatography applications. Here we analyzed the performance of the KNAUER PEEK pulse damper in combination with the compact pump AZURA P 4.1S. The pulse damper was able to reduce pressure pulsation by up to 92 %. The best working conditions for the pulse damper were determined at small flow rates in combination with a low back pressure within the system. Reducing the degree of pressure pulsation prevents potential damage to the column packing and therefore it is highly recommended to include the pulse damper within the flow path of the AZURA SEC FPLC system. Here, two system configurations are reviewed in detail.
VTN0013
Technical note, VTN0008
Technical note, VTN0007
Technical note, VTN0006
Information for analytical autosamplers. Technical note, VTN0004
Technical note, VTN0003
Serial pumps like piston pumps are the standard solution for eluent delivery in HPLC systems. Although they are very reliable and require low-maintenance, residual pulsation cannot be fully prevented. Technical note, VTN0002
Peak recycling elongates the separation length of the column. Technical note, VTN0001
