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Automated two-step purification of 6xHis-tagged GFP with AZURA Bio LC

Application No.: VBS0067

Automated two-step purification of 6xHis-tagged GFP with AZURA Bio LC

Affinity chromatography by His-Tag is one of the most widespread purification techniques for recombinant proteins. In many cases it requires an additional cleaning/polishing step. This application highlights the possibility of combining two subsequent chromatography protocols without manual interaction using the AZURA Bio LC system.

Technical Data

Application

Method FPLC

Separation conditions

Mode SEC
Substances Green fluorescence protein (GFP) with 6xHis - Tag
Key words GFP, His-Tag, IMAC, FPLC, affinity, SEC, buffer exchange
CAS number -

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