10 Things about biochromatography

Fast protein liquid chromatography (FPLC) of biomolecules can be a demanding task and is different from classical HPLC in many ways. This training video explains the main differences between HPLC and FPLC and shows the 10 things you need to know about biochromatography: 

  • differences in pressure
  • purity and yield
  • samples
  • native conditions
  • system compatibility
  • phases and materials
  • separation mechanisms
  • purification strategy 
  • analysis of the purified product
  • software