Eurosil Bioselect: ideal for large bio-molecules
The Eurosil Bioselect stationary phase was specifically developed for the separation of proteins and peptides. Eurosil Bioselect columns are available in 3 modifications, useful for a wide range of separation tasks involving large molecules up to 50 000 Da.
Strict manufacturing procedures ensure a very narrow pore size distribution. Interfering anions or heavy metal ions on the silica surface are not present in Eurosil Bioselect columns because only ultra pure silica gel and high quality chemical modifications are employed.
Physical properties of Eurosil Bioselect
Silica gel | ultra pure, >99.99 % |
Metal content | < 10 ppm |
Particle size | 3 μm, 5 μm, 10 μm, (15 μm, 20 – 25 μm upon request) |
Particle form | spherical |
Pore size | 300 Å |
Specific surface | 90 ± 5 m2/g |
Density | 0.45 ± 0.10 g/l |
Eurosil Bioselect columns for HPLC feature a wide-pore (300 Å) stationary phase and were specifically designed to separate large biomolecules, such as proteins and peptides.
- separation of proteins with MW > 10 000 Da
- available as C18, C18A, and C4 bonded phases
- excellent resolution with 3 μm particle size columns
- 3, 5, 10, and 15 μm particle sizes for easy scale-up
Modifications of Eurosil Bioselect
With our focused range of three different surface modifications, the most important applications in reversed phase mode biochromatography are covered. All Eurosil Bioselect modifications offer high chemical stability and high loading capacity. KNAUER’s long experience and knowledge in producing HPLC columns ensures you of excellent batch reproducibility.
Modification | USP code | Application | Carbon % | pH range |
|---|---|---|---|---|
C18 | L1 | hydrophilic biomolecules, peptides, peptide mapping after enzymatic digestion | 7.5 % (conventional encapping) | 2-8 |
C18 A | L1 | hydrophilic and polar biomolecules, peptides, very polar biomolecules separated with 100 % aqueous eluents | 7 % (conventional encapping) | 2-8 |
C18 | L1 | biomolecules such as proteins MW > 10 000 Da | 2 % (conventional encapping) | 2-8 |
Eurosil Bioselect applications
Eurosil Bioselect columns are particularly suitable for the separation of hydrophobic substances such as proteins. Very good results can also be obtained for separating oligonucleotides and for peptide mapping after enzyme digestion.
Click here for a list of all available Eurosil Bioselect columns
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Columns: Eurosil Bioselect brochure | (370 KB) |
