Bluespher: take HPLC to the next level with ultra high performance Bluespher columns

When you’re ready to switch your analytical HPLC method to UHPLC, Bluespher columns are the solution. Bluespher columns were developed to feature very similar characteristics and selectivities to the KNAUER Eurospher I and II families to make method transfer easy. Improve speed and resolution, and lower eluent consumption by scaling down to 2 mm ID Bluespher columns packed with 2 μm particles.

Bluespher columns are packed with ultra pure silica stationary phase to provide excellent separation performance and are well-suited for either routine analysis or ambitious chromatography in high speed mode where resolution, sensitivity and sample throughput are critical. These columns are your first choice for high-throughput-screening, quality control, and method development.

Physical properties of Bluespher

Silica gel	ultra pure, > 99.99 %
Particle size	2.0 μm
Particle form	spherical
Pore size	100 Å
Specific surface	320 ± 20 m2/g
Pore volumen	0.8 ml/g
Density	430 g/l

Bluespher offers outstanding mechanical and chemical stability for reliable performance.

Bluespher modifications

With a wide range of different surface modifications all the application fields of reversed phase chromatography are covered. By employing mono- and multi-functional silanes in the manufacturing process, every Bluespher type was designed to achieve a maximum in loading capacity and chemical stability.

Modification	Comparable selectivity to	USP code	Carbon %	pH range	Ordering code
C18 P	Eurospher II C18 P	L1	20% (> 99.9 % endcapping)	2-12	...E182BSF
C18 H	Eurospher II C18 H	L1	17% (> 99.9 % endcapping)	2-12	...E185BSF
C18	Eurospher I / II C18	L1	16% (~ 50 % endcapping)	2-8	...E181BSF
C18 A	Eurospher II C18 A	L1	10% (~ 50 % hydrophilic endc.)	2-8	...E184BSF
Phenyl	Eurospher II Phenyl	L11	12% (~ 50 % endcapping)	2-8	...E050BSF
C8	Eurospher I / II C8	L7	10% (~ 50 % endcapping)	2-8	...E081BSF
Si	Eurospher I / II Si	L3	0% (no endcapping)	2-8	...E000BSF

Scale up and scale down

Take advantage of the comparable selectivities of Eurospher, Eurospher II, and Bluespher columns to easily scale down your existing analytical method to UHPLC as well as to scale it up to semi-prep or even preparative LC.

Bluespher applications

Finding the appropriate column for a particular application can be a challenging task. With a range of bonded phases offering different selectivity, the Bluespher family includes columns to meet most separation needs. The chart below will help you to choose the best Bluespher column for a particular application.

Modification	Application areas	Separation mechanism
C18 P	alternative selectivity to standard C18; stationary phase with the highest carbon load of all Bluespher C18 modifications; fully endcapped; excellent shape selectivity and pH stability	hydrophobic and steric interaction
C18 H	recommended alternative for Kromasil 100 C18 columns; for acidic, basic and neutral analytes in reversed phase mode with extended pH range	hydrophobic interaction
C18	for acidic, basic and neutral analytes in reversed phase mode (sulphonamides; anabolic steroids; anti-psychotics; beta blocker; Sudan dyes; phenols, preservatives etc.)	hydrophobic interaction
C18 A	polar endcapped C18 phase for alternative selectivity; 100 % aqueous eluents for analysis of very polar compounds, basic pharmaceutical ingredients, water soluble vitamins, catecholamines as well as organic acids	hydrophobic and polar interaction
Phenyl	alternative selectivity for aromatic and moderately polar analytes or mixtures with varying polarity and aromaticity	π-π interaction with aromatics
C8	similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of water soluble vitamins, steroids, catecholamines sedatives etc.	reduced hydrophobic interaction comparing to C18 phase
Si	wide range of different applications, i.e. size exclusion chromatography) as well as normal phase HPLC; good choice for separations of polar compounds	hydrophilic interaction

Click here for a list of all available Bluespher columns

Your contact

Columns and applications department

Phone: +49 (0)30 809 727-0

Fax: +49 (0)30 801 50 10

columns@knauer.net Contactform

Selected applications with Bluespher columns

Determination of AQC Amino Acids with PDA (II)

Downloads

	Columns: Bluespher brochure	(327 KB)
	Columns: Bluespher operating guidelines	(62 KB)

Available Bluespher column kits

Products