The following selected scientific publications have been made by KNAUER scientists, in cooperation with KNAUER or using KNAUER HPLC systems or columns. Some of the publications may only be available on purchase.

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“High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications”

By Martin Zühlke, Daniel Riebe, Toralf Beitz, Hans-Gerd Löhmannsröben, Sandro Andreotti, Knut Reinert, Karl Zenichowski, Marc Diener

In: J. Sep. Sci. 2016, 39, 4756–4764

The complete article can be found at:

Keywords: Amino acids / Electrospray ionization / Ion mobility spectrometry /Pesticides / Two-dimensional separations

"Sensitive Online SPE Determination of Bisphenol A in Water Samples"

by Juliane Böttcher and  Mareike Margraf
in: The Analytical Scientist 44, September 2016, page 49

The complete article can be found at:

Key words: bisphenol A, BPA, drinking water analysis, solid-phase extraction, SPE

"FPLC versus Analytical HPLC: Two Methods, One Origin, Many Differences"

by Stephanie Runde
in: The Column, Volume 12, Issue 15, August 2016, page 12–16

The complete article can be found at:

Key words: FPLC, Bio LC, HPLC, biomolecules, size-exclusion, ion-exchange, affinity, IMAC

"Are the Lights on Green? - Micro-LC’s Road to Routine Analysis"

by Thorsten Teutenberg, Terence Hetzel, Denise Loeker, Juri Leonhardt, Philipp Schulze.
in: GIT Laboratory Journal, June 14 2016

The complete article can be found at:

Key words: nano LC, Micro LC, HPLC, fibre optics, extra column volume

"Characterization of commercially available peptidases in respect of the production of protein hydrolysates with defined compositions using a three-step methodology"

 by Michael Merz, Wolfgang Claaßen, Daniel Appel, Pieter Berends, Swen Rabe, Imre Blank, Timo Stressler, and Lutz Fischer.
in: Journal of Molecular Catalysis B: Enzymatic 127, May 2016, pages 1-10

The complete article can be purchased at: 

Key words: Commercial peptidases, peptidase composition, amino acid release; automated enzymatic hydrolysis; functional hydrolysates


"Sensitive Analysis of the Lactose Content of Lactose-Free Labelled Products Using HPAEC-PAD"

by Mareike Margraf and Dr. Kate Monks
in: LCGC Europe, The Applications Book, December 2015, page 680 f.

"Determination of Lactose in Lactose-free Products Using HPAEC-PAD",

by Mareike Margraf and Dr. Kate Monks
in: The Analytical Scientist, October 2015, page 57

Key words: High performance anion-exchange chromatography, pulsed amperometric detection, lactose intolerance, lactose thresholds, lactase enzyme, glucose, galactose, sugar content, dairy food analysis, electrochemical detection, trace analysis of carbohydrates

"An electrospray ionization-ion mobility spectrometer as detector for high-performance liquid chromatography"

by Martin Zühlke, Daniel Riebe, Toralf Beitz, Hans-Gerd Löhmannsröben, Karl Zenichowski, Marc Diener, and Michael W. Linscheid.
in: European Journal of Mass Spectrometry. Volume 21 Issue 3 (2015), Special Issue: Celebrating the 20th Anniversary of EJMS, pages 391–402

Key words: multijet electrospray, electrospray mechanism, source design, imaging, fast multidimensional chromatography, full HPLC flowrate compatibility, split-free electrospray source, ion-mobility separation, collision cross section measurement, separation of isomers, analysis of pesticides, neuroleptics and antibiotics

"Comparison of Compounds in Bourbon Vanilla Extract and Vanilla Flavour"

by Mareike Margraf
in: The Application Notebook, LC/Gc, Jul 01, 2015

Key words: 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin, natural Bourbon vanilla flavor, guaiacol, ethylvanillin, coumarin, eugenol, synthetic vanilla flavor, vanillin synthesis process, artificial vanilla flavour

"Automated solid-phase extraction coupled online with HPLC-FLD for the quantification of zearalenone in edible oil"

by Sarah S. Drzymala, Stefan Weiz , Julia Heinze, Silvia Marten, Carsten Prinz, Annett Zimathies, Leif-Alexander Garbe, Matthias Koch.
in: Analytical and Bioanalytical Chemistry, May 2015, Volume 407, Issue 12, pages 3489-3497

Key words: Dynamic covalent hydrazine chemistry (DCHC), maize germ oil, mycotoxin

"Innovative Chromatography System Revolutionizes Purification and Yield"

by S. Schagen, M. Fuchs, U. Maschke, S. Marten, A. Knauer, M. Bavand, and T. Müller-Späth
in: Global Ingredients & Formulations Guide 2015; Chapter VI - Production Technologies, pp. 156 – 162

Keywords: Multi-column chromatography, Continous Chromatography, MCSGP, FPLC, Capture SMB, Affinity Chromatography, Protein A column, mAb purification