Search:

High resolution glass columns

The glass columns offer highest pressure stability and a wide temperature range, which makes them suitable for most biochromatograpy applications. They can be packed and refilled with any BioFox separation media for your bioseparations and purifications of proteins: affinitiy chromatography, ion-exchange chromatography, immobilized metal ion affinity chromatography (IMAC) and size exclusion chromatography (SEC).

Available Glass columns

glass column

ID

10 mm

20 mm

30 mm

50 mm

100 mm

Length

30 cm

A71110

Max. volume: 15.7 ml

A71120

Max. volume: 62 ml

A71130

Max. volume: 141 ml

A71150

Max. volume: 392 ml

*available soon*

60 cm

A71118

Max. volume: 39 ml

A71115

Max. volume: 157 ml

A71116

Max. volume: 353 ml

A71151

Max. volume: 980 ml

*available soon*

100 cm

A71119

Max. volume: 70 ml

A71222

Max. volume: 282 ml

A71117

Max. volume: 636 ml

Glass column features:

  • chemically and biologically inert borosilicate glass
  • pressure resistant columns allow for higher flow rates and faster separations
  • glass columns are available in 3 lengths and 3 IDs for a wide range of applications and easy upscaling
  • axial compression and column bed height adjustment via a variable adaptor with integrated quick release lever
  • "Uniform flow" distributor on both adaptors makes reverse flow ("Up / Down Flow") possible
  • thermostatting jacket is resistant to cooling liquid
  • easy to change PEEK and PTFE frits
  • robust, pressure resistant design of funnel and fingertight adaptors for safe handling, ease-of-use and longevity
  • all column components - including the glass column and flexible and fixed adaptors - are also available individually

Technical data

Max. height of gel bed

column length minus 100 mm

Maximum pressure

columns with 10 and 20 mm ID: 100 bar (10 Mpa)

columns with 30 mm ID: 80 bar (8 Mpa)

columns with 50 mm ID: 25 bar (2.5 Mpa)

Temperature range

4 - 60 °C

Column tube

borosilicate glass

resistant against buffers and acids as well as HPLC solvents

not for use with hydrofluoric or phosphoric acid, nor strong alkaline substances

Heating / cooling jacket

wetted material: PMMA (poly(methylmethacyrlate), Plexiglas®)

for use with water + 10 % methanol (little corrosion with 100 % methanol)

Adaptor head and flow distributor

PEEK

resistant against buffers and acids as well as HPLC solvents

Adapter frits

Columns with 10 and 20 mm ID: PEEK, 10 μm
resistant against buffers and acids as well as HPLC solvents
Columns with 30 and 50 mm ID: PTFE, 10 μm
resistant against buffers, methanol and acetone, do not use with acetonitrile

a 5 µm PTFF frit is available on request

Adapter shaft

stainless steel (not in contact with eluent)

Capillaries, nuts, ferrules

columns with 10, 20 and 30 mm ID: PEEK, 1/16"

columns with 50 mm ID: PEEK, 1/8"

resistant against buffers and acids as well as HPLC solvents

Your contact

Wissenschaftliche Gerätebau
Dr. Ing. Herbert Knauer GmbH
Hegauer Weg 38
14163 Berlin
Phone: +49 30 809 727 0
Fax: +49 30 8015010
biochromatography@knauer.net Contact us