Classical C18 columns for Reversed Phase in HPLC and UHPLC (USP L1)
Most often used stationary phase used in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
C18 columns are ideally used for acidic, basic and neutral analytes in reversed phase mode, e.g.:
- organic acids
- anabolic steroids
- beta blocker
- Sudan dyes