HILIC HPLC Columns
HILIC or Hydrophilic Interaction Liquid Chromatography is based on Partition chromatography between mobile and stationary phase.
Typical analytes: Hydrophilic compounds that are poorly or not retained in Reversed Phase and cannot be analyzed in Normal Phase because they are water soluble and unsoluble in NP solvents. For example:
- Polar organic acids and bases
- Natural polar compounds
- Amino acids
- Water soluble vitamins