HILIC HPLC Columns

HILIC or Hydrophilic Interaction Liquid Chromatography is based on Partition chromatography between mobile and stationary phase.

Typical analytes: Hydrophilic compounds that are poorly or not retained in Reversed Phase and cannot be analyzed in Normal Phase because they are water soluble and unsoluble in NP solvents. For example:

  • Polar organic acids and bases
  • Natural polar compounds
  • Nucleosides
  • Oligonucleotides
  • Amino acids
  • Peptides
  • Water soluble vitamins
Vertex Plus columns, 30x2 mm, 50x2 mm, 100x2 mm, 150x2 mm

Si

USP L3

NH2

USP L8

zwitterionic HILIC

Eurospher II HILIC

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