1. 10-Deacetyl baccatin; 2. Baccatin III; 3. 10-Deacetyl-7-xylosyl taxol B; 4. Taxinine M; 5. 10-Deacetyl-7-xylosyl taxol; 6. 10-Deacetyl taxol; 7. 10-Deacetyl-7-xylosyl taxol C; 8. 7-Xylosyl taxol; 9. Cephalomanine; 10. 10-Deacetyl-7-epitaxol; 11. Paclitaxel; 12. Taxol C; 13. 7-Epitaxol
Separation of taxanes (I)
Article number: VPH0041J
| Method | HPLC/UHPLC |
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| Eluent | A: Water B: Methanol/Acetonitrile 7:93 (v/v)
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| Gradient | 0 – 1.2 min 35 % B 1.2 – 4 min 58 % B 4 – 5 min 58 % B (2 min)
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| Flow rate | 0.6 ml/min |
| Mode | RP-Mode |
| Injection volume | 2 µl |
| Temperature | 30 °C |
| Detection | PDA-1, 220 nm (100 Hz, 0.005 s) 10 mm 2 µl flow cell
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| Chromatogram | 1. 10-DEA (Diethylamine)cetyl baccatin 2. Baccatin III 3. 10-DEA (Diethylamine)cetyl-7-xylosyl taxol B 4. Taxinine M 5. 10-DEA (Diethylamine)cetyl-7-xylosyl taxol 6. 10-DEA (Diethylamine)cetyl taxol 7. 10-DEA (Diethylamine)cetyl-7-xylosyl taxol C 8. 7-Xylosyl taxol 9. Cephalomanine 10. 10-DEA (Diethylamine)cetyl-7-epitaxol 11. Paclitaxel 12. Taxol C 13. 7-Epitaxol
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Chromatogram VPH0041J | 1. 10-Deacetyl baccatin; 2. Baccatin III; 3. 10-Deacetyl-7-xylosyl taxol B; 4. Taxinine M; 5. 10-Deacetyl-7-xylosyl taxol; 6. 10-Deacetyl taxol; 7. 10-Deacetyl-7-xylosyl taxol C; 8. 7-Xylosyl taxol; 9. Cephalomanine; 10. 10-Deacetyl-7-epitaxol; 11. Paclitaxel; 12. Taxol C; 13. 7-Epitaxol |
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Vertex Plus Column 50 x 2 mm BlueOrchid 175-1.8 PFP | 05BI057BOE | Vertex Plus Column 50 x 2 mm BlueOrchid 175-1.8 PFP |
Technical data are subject to change without notice.