HPLC

A: Acetonitrile

B: 0.2 g NaH₂PO₄ in 1000 ml Water (pH 4 with H₃PO₄)

0 – 3.5 min 22 % A

3.5 – 4.5 min 22% – 30% A

4.5 – 6 min 30% – 90% A

6 – 8 min 90% A

0 – 3.5 min 0.2 ml/min

3.5 – 10 min 0.4 ml/min

RP Mode

10 µl

40 °C

UV at 265 nm

Sulfadiazine, Sulfamethiazole, Sulfamethoxypyridazine, Sulfamethoxazole, Sulfadimethoxine

Sulfa drugs, antibiotics

1. Sulfadiazine

2. Sulfamethiazole

3. Sulfamethoxypyridazine

4. Sulfamethoxazole

5. Sulfadimethoxine