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1. Impurity 1; 2. 4-Phenylazophenol; 3. a-Naphthyl red; 4. Impurity 2; 5. Impurity 3; 6. Sudan II; 7. Sudan III; 8. Sudan IV

Separation of Sudan dyes

Article number: VFD0076J

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Details
Method

HPLC/UHPLC

Eluent

A: Water (+ 0.1 % formic acid)

B: Acetonitrile (+ 0.1 % formic acid)

 

Gradient

0 – 0.5 min 70 % B

0.5 – 1.5 min 100 % B

1.5 – 2.0 min 100 % B

 

Flow rate

1.0 ml/min

Mode

RP-Mode

Injection volume

1 µl

Pressure

400 bar (5800 psi)

Temperature

25 °C

Detection

PDA-1, 240 nm (50 Hz, 0.01 s)

10 mm, 2.4 µl flow cell

 

Chromatogram

1. Impurity 1

2. 4-Phenylazophenol

3.α-Naphthyl red

4. Impurity 2

5. Impurity 3

6. Sudan II

7. Sudan III

8. Sudan IV

 

Downloads
  Title asc Description asc Languages
Chromatogram VFD0076J Chromatogram VFD0076J 1. Impurity 1; 2. 4-Phenylazophenol; 3. a-Naphthyl red; 4. Impurity 2; 5. Impurity 3; 6. Sudan II; 7. Sudan III; 8. Sudan IV
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