HPLC

A: Methanol / Water (80:20)

B: Acetonitrile / THF (93:7)

0 – 15 min 0 – 100% B

15 – 20 min 100% B

equilibrate column 10 min with 100 % A for next injection

0.2 ml/min

RP Mode

5 µl

30 °C

Fluorescence, wavelength program (excitation / emission):

1. 275/350 nm, 2. 260/390 nm, 3. 260/420 nm, 4. 290/430 nm, 5. 300/500 nm

UV at 254 nm

Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Pyrene, Benzo(a)anthracene, Chrysene, Benzo(b)fluoranthene, Benzo(k)fluoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene, Benzo(g,h,i)perylene, Indeno(1,2,3-cd)pyren

16 PAH´s according to EPA (550.1 / 610 / 8310), Aromatic compounds

1. Naphthalene

2. Acenaphthylene

3. Acenaphthene

4. Fluorene

5. Phenanthrene

6. Anthracene

7. Fluoranthene

8. Pyrene

9. Benzo(a)anthracene

10. Chrysene

11. Benzo(b)fluoranthene

12. Benzo(k)fluoranthene

13. Benzo(a)pyrene

14. Dibenzo(a,h)anthracene

15. Benzo(g,h,i)perylene

16. Indeno(1,2,3-cd)pyrene