1. 3,4-Dihydroxyphenylacetic acid; 2. D,L-tryptophan; 3. Homovanillic acid
Separation of D,L-Tryptophan and Aromatic acids
Article number: VBS0009J
Details
| Method | HPLC |
|---|---|
| Eluent | Methanol / Water (30:70) with 6 g/l Ammonium acetate and 1.5 g/l Sulphuric acid
|
| Gradient | isocratic |
| Flow rate | 0.7 ml/min |
| Mode | RP Mode |
| Injection volume | 10 µl (40 ng each) |
| Temperature | 25 °C |
| Detection | Amperometric detection, +1000 mV / 800 mV, range 100 nA |
| Substances | Dihydroxyphenylacetic acid, Tryptophan, Homovanillic acid |
| Key words | Biogenic amines, Aromatic acids |
| Chromatogram | 1. 3,4-Dihydroxyphenylacetic acid 2. D,L-tryptophan 3. Homovanillic acid
|
Downloads
Title
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Description
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Chromatogram VBS0009J | 1. 3,4-Dihydroxyphenylacetic acid; 2. D,L-tryptophan; 3. Homovanillic acid |
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VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 | 25DE181ESJ | VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.
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