Overlay of the AQC-AAA standard and a derivatized sample
1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. ? (Derivatization); 6. Cys; 7. His; 8. Thr; 9. Arg; 10. Ala; 11. AMQ (Derivatization); 12. Pro; 13. NH3; 14. Lys; 15. Tyr; 16. Val; 17. Met; 18. Ile; 19. Leu; 20. Cys Peak 2; 21. Phe
Separation of AQC Derivatized Amino Acids with Fluorescence Detection
Article number: VFD0064J
| Method | HPLC |
|---|---|
| Eluent | A: 50 mM Na-Acetat pH 5.75 B: 50 mM Na-Acetat pH 6/Acetonitrile 30:70 |
| Gradient | 0 – 10.13 min 5 % – 10 % B 10.13 – 16.0 min 10 % B – 25 % B 16.0 – 21.9 min 25 – 32 % B
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| Flow rate | 0.8 ml/min |
| Mode | Matrix |
| Injection volume | 1 µl (1.176 pmol/µl, Cys 0.588 pmol/µl) |
| Temperature | 45 °C |
| Detection | Fluorescence (Ex 250 nm, Em 395 nm) |
| Substances | Alanine, Arginine, Aspartic acid, Cysteine, Glutamic acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, Valine |
| Key words | Amino acids, AQC |
| Chromatogram | 1. Asp 2. Glu 3. Ser 4. Gly 5. ? (Derivatization) 6. Cys 7. His 8. Thr 9. Arg 10. Ala 11. AMQ (Derivatization) 12. Pro 13. NH3 14. Lys 15. Tyr 16. Val 17. Met 18. Ile 19. Leu 20. Cys Peak 2 21. Phe
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Chromatogram VFD0064J |
Overlay of the AQC-AAA standard and a derivatized sample 1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. ? (Derivatization); 6. Cys; 7. His; 8. Thr; 9. Arg; 10. Ala; 11. AMQ (Derivatization); 12. Pro; 13. NH3; 14. Lys; 15. Tyr; 16. Val; 17. Met; 18. Ile; 19. Leu; 20. Cys Peak 2; 21. Phe |
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VertexPlus Column 150 x 3 mm Eurospher II 100-3 C18 | 15CE181E2G | VertexPlus Column 150 x 3 mm Eurospher II 100-3 C18 |
Technical data are subject to change without notice.