1. o-Toluidine; 2. Sulfanilamide; 3. Sulfmethoxydiazone
Separation of Anilines and Sulfonamides by HPLC with electrochemical detection
Article number: VPH0012J
Details
| Method | HPLC |
|---|---|
| Eluent | Methanol / Water (1:1) with 2 g/l KNO3 and 0.05 g/l Sulfuric acid |
| Gradient | isocratic |
| Flow rate | 0.7 ml/min |
| Mode | RP Mode |
| Injection volume | 10 µl |
| Temperature | 25 °C |
| Detection | Amperometric detection, glassy carbon electrode, range 100 nA +1000 mV for o-Toluidine / 1200 mV for sulfonamides
|
| Substances | Toluidine-o, Sulfanilamide, Sulfamethoxydiazine |
| Key words | Drugs, Antibiotics |
| Chromatogram | 1. o-Toluidine 2. Sulfanilamide 3. Sulfmethoxydiazone
|
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Chromatogram VPH0012J | 1. o-Toluidine; 2. Sulfanilamide; 3. Sulfmethoxydiazone |
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VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 | 25DE181ESJ | VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.
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