1. Ethanolamine,; 2. Ammonia,; 3. Methylamine,; 4. Serotonin,; 5. Ethylamine,; 6. Morpholine,; 7. Isopropylamine,; 8. Dimethylamine,; 9. Propylamine,; 10. Pyrrolidine,; 11. 2-Pyrrolidone,; 12. Octopamine,; 13. Indole,; 14. Butylamine,; 15. Diethylamine,; 16. Phenethylamine,; 17. 2-Methylbutylamine,; 18. Isopentylamine,; 19. Pentylamine,; 20. Piperidine,; 21. 1,3-Diaminopropane,; 22. 1,4-Diaminobutane,; 23. Hexylamine,; 24. 1,5-Diaminopentane,; 25. Histamine,
Separation of Amines in wine with precolumn derivatization (DANSYL)
Article number: VFD0025J
| Method | HPLC |
|---|---|
| Eluent | Acetonitrile / Water (30:70) |
| Gradient | over 40 min to 60% Acetonitrile and 15% Ethanol in water, 10 min hold |
| Flow rate | 1.0 ml/min |
| Mode | RP Mode |
| Injection volume | 20 µl |
| Temperature | ambient |
| Detection | Fluorescence: excitation 338 nm, emission 455 nm |
| Substances | Ethanolamine, Ammonia, Methylamine, Serotonin, Ethylamine, Morpholine, Isopropylamine, Dimethylamine, Propylamine, Pyrrolidine, Pyrrolidone, Octopamine, Indole, Butylamine, Diethylamine, Phenethylamine, Methylbutylamine, Isopentylamine, Pentylamine, Piperidine, Diaminopropane, Diaminobutane, Hexylamine, Diaminopentane, Histamine |
| Key words | Amines, Precolumn derivatization, DANSYL |
| Chromatogram | 1. Ethanolamine 2. Ammonia 3. Methylamine 4. Serotonin 5. Ethylamine 6. Morpholine 7. Isopropylamine 8. Dimethylamine 9. Propylamine 10. Pyrrolidine 11. 2-Pyrrolidone 12. Octopamine 13. Indole 14. Butylamine 15. Diethylamine 16. Phenethylamine 17. 2-Methylbutylamine 18. Isopentylamine 19. Pentylamine 20. Piperidine 21. 1,3-Diaminopropane 22. 1,4-Diaminobutane 23. Hexylamine 24. 1,5-Diaminopentane 25. Histamine
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Chromatogram VFD0025J | 1. Ethanolamine,; 2. Ammonia,; 3. Methylamine,; 4. Serotonin,; 5. Ethylamine,; 6. Morpholine,; 7. Isopropylamine,; 8. Dimethylamine,; 9. Propylamine,; 10. Pyrrolidine,; 11. 2-Pyrrolidone,; 12. Octopamine,; 13. Indole,; 14. Butylamine,; 15. Diethylamine,; 16. Phenethylamine,; 17. 2-Methylbutylamine,; 18. Isopentylamine,; 19. Pentylamine,; 20. Piperidine,; 21. 1,3-Diaminopropane,; 22. 1,4-Diaminobutane,; 23. Hexylamine,; 24. 1,5-Diaminopentane,; 25. Histamine, |
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VertexPlus Column 250 x 4.6 mm Eurospher 100-5 C18 | 25EE181ESJ | VertexPlus Column 250 x 4.6 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.