Chromatograms of extracts from; a) blank plasma; b) plasma spiked with:; 1. 250 ng/ml metabolite; 2. 250 ng/ml amineptine; 3. 500 ng/ml internal standard; c) a patient plasma sample; obtained 45 min after taking a; 100 mg oral dose of; amineptine, spiked with; 1. 230 ng/ml metabolite; 2. 180 ng/ml amineptine; 3. 500 ng/ml internal standard
HPLC method for the determination of Amineptine and its main Metabolite in human plasma
Article number: VPH0009J
| Method | HPLC |
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| Eluent | Aqueous phase containing 1.2 g/l heptane sulphonate adjusted to pH 3.0 with H3PO4 / Acetonitrile (62:38)
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| Gradient | isocratic |
| Flow rate | 1.0 ml/min |
| Mode | RP Mode (Ion pair) |
| Injection volume | 20 µl |
| Temperature | 30 °C |
| Detection | UV at 220 nm |
| Substances | Amineptine |
| Key words | Drugs, Antidepressant drugs |
| Chromatogram | Chromatograms of extracts from a) blank plasma b) plasma spiked with: 1. 250 ng/ml metabolite 2. 250 ng/ml amineptine 3. 500 ng/ml internal standard c) a patient plasma sample obtained 45 min after taking a 100 mg oral dose of amineptine, spiked with 1. 230 ng/ml metabolite 2. 180 ng/ml amineptine 3. 500 ng/ml internal standard
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Chromatogram VPH0009J | Chromatograms of extracts from; a) blank plasma; b) plasma spiked with:; 1. 250 ng/ml metabolite; 2. 250 ng/ml amineptine; 3. 500 ng/ml internal standard; c) a patient plasma sample; obtained 45 min after taking a; 100 mg oral dose of; amineptine, spiked with; 1. 230 ng/ml metabolite; 2. 180 ng/ml amineptine; 3. 500 ng/ml internal standard |
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VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18 | 15EE181ESJ | VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.