1. Histamine; 2. Tyramine; 3. Putrescine; 4. Phenethylamine; 5. Cadaverine; 6. Isoamylamine; 7. internal Standard IS
HPLC determination of Amines in wine with precolumn derivatization (OPA)
Article number: VFD0026J
Details
| Method | HPLC |
|---|---|
| Eluent | 0.08 M Acetic acid / Acetonitrile (70:30) 3 min |
| Gradient | in 6.5 min to 65% Acetonitrile, in 11 min to 72% Acetonitrile, in 1 min to 80% Acetonitrile, 3 min hold
|
| Flow rate | 1.0 ml/min |
| Mode | RP Mode |
| Injection volume | 20 µl |
| Temperature | ambient |
| Detection | Fluorescence: excitation 230 nm, emission 440 nm |
| Substances | Cadaverine, Heptylamine, Histamine, Isoamylamine, Pentyl-i-amine, Phenethylamine, Putrescine |
| Key words | Amines, Precolumn derivatization , OPA |
| Chromatogram | 1. Histamine 2. Tyramine 3. Putrescine 4. Phenethylamine 5. Cadaverine 6. Isoamylamine 7. internal Standard IS
|
Downloads
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Chromatogram VFD0026J | 1. Histamine; 2. Tyramine; 3. Putrescine; 4. Phenethylamine; 5. Cadaverine; 6. Isoamylamine; 7. internal Standard IS |
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VertexPlus Column 250 x 4.6 mm Eurospher 100-5 C18 | 25EE181ESJ | VertexPlus Column 250 x 4.6 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.
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