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1. Ribonuclease A; 2. Insulin bovine; 3. Lysozyme; 4. BSA; 5. Myoglobin; 6. Ovalbumin; 7. Not identified

Fast separation of Proteins I

Article number: VBS0007J

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Details
Method

HPLC

Eluent

A: Water / 0.1% TFA (Trifluoroacetic acid)

B: Acetonitrile / Water (95:5) + 0.1% TFA (Trifluoroacetic acid)

 

Gradient

0 – 25 s 29% – 48% B

25 – 65 s 48% – 100% B

 

Flow rate

4.0 ml/min

Mode

RP Mode

Injection volume

5 µl

Temperature

30 °C

Detection

UV at 220 nm

Substances

BSA, Insulin bovine, Lysozyme, Myoglobin, Ovalbumin, Ribonuclease A

Key words

Proteins

Chromatogram

1. Ribonuclease A

2. Insulin bovine

3. Lysozyme

4. BSA

5. Myoglobin

6. Ovalbumin

7. Not identified

 

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Chromatogram VBS0007J Chromatogram VBS0007J 1. Ribonuclease A; 2. Insulin bovine; 3. Lysozyme; 4. BSA; 5. Myoglobin; 6. Ovalbumin; 7. Not identified
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