HPLC

A: Water B: Methanol

0 – 19.7 min 50 % – 95 % B

19.7 – 20.7 min 95 % B

20.7 – 22 min 95 % – 50 % B

0.6 ml/min

RP Mode

1 µl

40 °C

UV at 254 nm (10 mm cell, 5 Hz, 0.2 s)

Cortisone, Corticosterone, Desoxycorticosterone, Testosterone, Norgestrel, Progesterone

Steroids, Steroid hormones, Pharmaceutical

1. Cortisone

2. Corticosterone

3. Desoxycorticosterone

4. Testosterone

5. Norgestrel

6. Progesterone