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1. 4-Hydroxy benzoic acid; 2. 2-Methoxy benzoic acid; 3. Benzoic acid; 4. Sorbic acid; 5. p-Hydroxy methyl benzoate; 6. p-Hydroxy ethyl benzoate; 7. p-Hydroxy propyl benzoate; 8. p-Hydroxy butyl benzoate

Determination of preservatives

Article number: VFD0075J

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Details
Method

HPLC/UHPLC

Eluent

A: Ammonium formiate buffer/

Methanol 70:30

B: Ammonium formiate buffer/

Methanol 40:60

 

Gradient

0 – 0.1 min 100 % A

0.1 – 0.5 min 100 % – 60 % A

0.5 – 1.2 min 60 % A

1.2 – 2 min 0 % A

 

Flow rate

0.85 ml/min

Mode

RP-Mode

Injection volume

0.5 µl

Temperature

40 °C

Detection

PDA-1, 240 nm (50 Hz, 0.02 s)

10 mm, 2 µl flow cell

 

Chromatogram

1. 4-Hydroxy benzoic acid

2. 2-Methoxy benzoic acid

3. Benzoic acid

4. Sorbic acid

5. p-Hydroxy methyl benzoate

6. p-Hydroxy ethyl benzoate

7. p-Hydroxy propyl benzoate

8. p-Hydroxy butyl benzoate

 

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Chromatogram VFD0075J Chromatogram VFD0075J 1. 4-Hydroxy benzoic acid; 2. 2-Methoxy benzoic acid; 3. Benzoic acid; 4. Sorbic acid; 5. p-Hydroxy methyl benzoate; 6. p-Hydroxy ethyl benzoate; 7. p-Hydroxy propyl benzoate; 8. p-Hydroxy butyl benzoate
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