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1. Gallic acid, 2. Chlorogenic acid, 3. Catechin, 4. Epicatechin, 5. Rutin, 6. Kaempferol-3-o-rutinoside, 7. Myricitin, 8. Quercetin

Determination of Polyphenols

Article number: VFD0082J

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Details
Method

HPLC

Sample

Eurospher II 100-3 C18 H, 150 x 3.0 mm ID

Separation column

Eurospher II 100-3 C18 H

Eluent

A: Water + 0.1 % Formic acid

B: Water + 0.1 % Formic acid/Acetonitrile 50:50 v/v

Gradient

0.0 – 2.0 min 10 % B

2.0 – 8.1 min 10 – 100 % B

8.1 – 10.1 min 100 % B

Flow rate

1.0 ml/min

Mode

Matrix

Injection volume

5 µl

Detection

UV at 280 nm

Substances

Catechin, Chlorogenic acid, Epicatechin, Gallic acid, Kaempferol-3-o-rutinoside, Myricitin, Quercetin, Rutin

Key words

Polyphenols

Chromatogram

1. Gallic acid

2. Chlorogenic acid

3. Catechin

4. Epicatechin

5. Rutin

6. Kaempferol-3-o-rutinoside

7. Myricitin

8. Quercetin

 

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Chromatoram VFD0082J 1. Gallic acid, 2. Chlorogenic acid, 3. Catechin, 4. Epicatechin, 5. Rutin, 6. Kaempferol-3-o-rutinoside, 7. Myricitin, 8. Quercetin
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Technical data are subject to change without notice.

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