HPLC

A: 814.2 ml n-Hexan, 170 ml 2-Propanol, 15 ml Acetic acid, 0.8 ml Triethylamine

B: 844.2 ml 2-Propanol, 140 ml Water, 15 ml Acetic acid, 0.8 ml Triethylamine

0% B – 20% B 0 – 5 min; 20 – 80% B 5 – 60 min (5 min hold)

1.0 ml/min (column reequilibration at 2 ml/min)

NP Mode

20 µl

55 °C

ELSD (50 °C, 2.5 bar N2)

Phosphatidylcholine (PC) , Phosphatditylethanolamine (PE) , Phosphatidylinositol (PI)

Phospholipids, ELSD, Lecitine

1. Phosphatditylethanolamine (PE)

2. Phosphatidylcholine (PC)

3. Phosphatidylinositol (PI)