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Determination of Patulin in apple juice

Article number: VFD0055J

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Details
Method

HPLC

Sample

extraction of apple juice with ethyl acetate (3x), extraction of the combined phases with Na2CO3 solution, extraction of the separated aqueous phase with ethyl acetate, addition of glacial acetic acid, reduction of ethyl acetate, redissolution of the dry residue in mobile phase

Eluent

Acetonitrile / Water (3:97)

Gradient

isocratic

Flow rate

1.0 ml/min

Mode

RP Mode

Injection volume

10 µl

Temperature

25 °C

Detection

UV (DAD) at 275 nm

Substances

Patulin

Key words

Mycotoxins

Downloads
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Chromatogram VFD0055J Chromatogram VFD0055J
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Technical data are subject to change without notice.

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