1. 4-Aminophenol; 2. Paracetamol; 3. N-(2-hydroxyphenyl)acetamide; 4. N-(4-hydroxyphenyl)propanamide; 5. N-phenylacetamide; 6. 1-(4-hydroxyphenyl)ethanone oxime; 7. 1-(2-hydroxyphenyl)ethanone; 8. Chloracetanilide; 9. 4-Nitrophenol
Determination of Paracetamol and by-products
Article number: VPH0025J
| Method | HPLC |
|---|---|
| Eluent | A: Acetonitrile B: Water (pH 2.75 with H3PO4) |
| Gradient | 0 – 0.65 min 10% A 0.65 – 6.4 min 10% – 60% A 6.4 – 10.4 min 60% A 10.4 – 12 min 60% – 10% A
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| Flow rate | 1.3 ml/min |
| Mode | RP Mode |
| Injection volume | 5µl |
| Temperature | 50 °C |
| Detection | UV at 245 nm |
| Substances | Paracetamol, N-(2-hydroxyphenyl)acetamide, N-(4-hydroxyphenyl)propanamide, N-phenylacetamide, Chloracetanilide, 1-(4-hydroxyphenyl)ethanone oxime, 1-(2-hydroxyphenyl)ethanone, 4-Aminophenol, 4-Nitrophenol |
| Key words | Paracetamol, Drugs |
| Chromatogram | 1. 4-Aminophenol 2. Paracetamol 3. N-(2-hydroxyphenyl)acetamide 4. N-(4-hydroxyphenyl)propanamide 5. N-phenylacetamide 6. 1-(4-hydroxyphenyl)ethanone oxime 7. 1-(2-hydroxyphenyl)ethanone 8. Chloracetanilide 9. 4-Nitrophenol
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Chromatogram VPH0025J | 1. 4-Aminophenol; 2. Paracetamol; 3. N-(2-hydroxyphenyl)acetamide; 4. N-(4-hydroxyphenyl)propanamide; 5. N-phenylacetamide; 6. 1-(4-hydroxyphenyl)ethanone oxime; 7. 1-(2-hydroxyphenyl)ethanone; 8. Chloracetanilide; 9. 4-Nitrophenol |
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VertexPlus Column 250 x 3 mm ProntoSIL 120-5 C8 ace-EPS | 25CF08APSJ | VertexPlus Column 250 x 3 mm ProntoSIL 120-5 C8 ace-EPS |
Technical data are subject to change without notice.