HPLC

fungus

A: Acetonitrile

B: 0.01 M Acetic acid

0 – 16 min 10% A

16 – 22 min 10% – 90% A

22 – 40 min 90% – 10% A

0.4 ml/min

up to 200 µl

25 °C

2 channel postcolumn derivatization, flow rate of 0.25 ml/min, temperature of 115 °C

Reactor Coil 1 : 1.2 ml, 0.15 M sodium hydroxide

Reactor Coil 2 : 1.6 ml, 0.03 M methyl acetoacetate and 2 M ammonium acetate

Fluorescence: excitation at 360 nm, emission at 470 nm

Deoxynivalenol (DON), Nivalenol (NIV)

Mycotoxins, Deoxynivalenol (DON), Nivalenol (NIV)

1. Nivalenol (NIV)

2. Deoxynivalenol (DON)