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1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 ?, 6 Lys, 7 His, 8 Thr, 9 Arg, 10 Ala, 11 AMQ, 12 Pro, 13 NH3, 14 Cys, 15 Tyr, 16 Val, 17 Met, 18 Ile, 19 Leu, 20 Lys 2, 21 Phe

Determination of AQC Amino Acids with PDA (II)

Article number: VBS0012J

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1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 ?, 6 Lys, 7 His, 8 Thr, 9 Arg, 10 Ala, 11 AMQ, 12 Pro, 13 NH3, 14 Cys, 15 Tyr, 16 Val, 17 Met, 18 Ile, 19 Leu, 20 Lys 2, 21 Phe


Details
Method

UHPLC

Eluent

A: 50 mM Sodium-Ac. pH 5.75
B: 30 % 50 mM Sodium-Ac. pH 6
    70 % ACN

Gradient

0 – 3 min 5 - 10 % B
3 – 4.75 min 10 - 25 % B
4.75 – 6.5 min 25 - 32 % B

Flow rate

0.8 ml/min

Injection volume

10 μl (11.76 pmol/ul)

Temperature

45 °C

Detection

PDA-1, 254 nm (10 mm cell, 50 Hz, 0.02 s)

Pressure

approx. 660 bar

Downloads
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Chromatogramm VVBS0012J 1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 ?, 6 Lys, 7 His, 8 Thr, 9 Arg, 10 Ala, 11 AMQ, 12 Pro, 13 NH3, 14 Cys, 15 Tyr, 16 Val, 17 Met, 18 Ile, 19 Leu, 20 Lys 2, 21 Phe
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