1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 Taurin, 6 Lys (+1 AMQ), 7 Thr, 8 His, 9 Ala, 10 Arg, 11 Pro, 12 CysCys, 13 Tyr, 14 Val, 15 Met, 16 Ile, 17 Leu, 18 Lys (+2 AMQ), 19 Phe, 20 Trp
Determination of AQC Amino Acids with MS detection
Article number: VBS0013J1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 Taurin, 6 Lys (+1 AMQ), 7 Thr, 8 His, 9 Ala, 10 Arg, 11 Pro, 12 CysCys, 13 Tyr, 14 Val, 15 Met, 16 Ile, 17 Leu, 18 Lys (+2 AMQ), 19 Phe, 20 Trp
| Eluent | A: 2.5 mM NH4-Ac. pH 5.75 B: 30 % 2.5 mM NH4-Ac. pH 6 and 70 % ACN |
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| Gradient | 0 – 3 min 5 - 10 % B 3 – 4.75 min 10 - 25 % B 4.75 – 6.5 min 25 - 32 % B |
| Flow rate | 0.8 ml/min |
| Injection volume | 10 µl (11.76 pmol/ul) |
| Temperature | 45 °C |
| Detection | MSQ Plus, ESI mode, Needle 3kV, coneV 75 V, Probe temperature 350 °C selected SIM Scans, positive ion mode (blue) negative ion mode (green) |
| Substances | 1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 Taurin, 6 Lys (+1 AMQ), 7 Thr, 8 His, 9 Ala, 10 Arg, 11 Pro, 12 CysCys, 13 Tyr, 14 Val, 15 Met, 16 Ile, 17 Leu, 18 Lys (+2 AMQ), 19 Phe, 20 Trp |
| Chromatogram | positive ion mode (blue) negative ion mode (green) 1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 Taurin, 6 Lys (+1 AMQ), 7 Thr, 8 His, 9 Ala, 10 Arg, 11 Pro, 12 CysCys, 13 Tyr, 14 Val, 15 Met, 16 Ile, 17 Leu, 18 Lys (+2 AMQ), 19 Phe, 20 Trp |
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Chromatogramm VVBS0013J | 1 Asp, 2 Glu, 3 Ser, 4 Gly, 5 Taurin, 6 Lys (+1 AMQ), 7 Thr, 8 His, 9 Ala, 10 Arg, 11 Pro, 12 CysCys, 13 Tyr, 14 Val, 15 Met, 16 Ile, 17 Leu, 18 Lys (+2 AMQ), 19 Phe, 20 Trp |
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Vertex Plus Column 100 x 2 mm Bluespher 100-2 C18 | 10BE181BSF | Vertex Plus Column 100 x 2 mm Bluespher 100-2 C18 |
Technical data are subject to change without notice.