1. Asp, 2. Glu, 3. Ser, 4. Gly, 5. ?, 6. Lys, 7. His, 8. Thr, 9. Arg, 10. Ala, 11. AMQ, 12. Pro, 13. NH3, 14. CysCys, 15. Tyr, 16. Val, 17. Met, 18. Ile, 19. Leu, 20. Lys 2, 21. Phe
Determination of AQC Amino Acids (II)
Article number: VBS0011J
Details
| Method | HPLC/UHPLC |
|---|---|
| Eluent | A: 50 mM Sodium-Ac. pH 5.75 B: 30 % 50 mM Sodium-Ac. pH 6, 70 % ACN |
| Gradient | 0 – 3 min 5 - 10 % B 3 – 4.75 min 10 - 25 % B 4.75 – 6.5 min 25 - 32 % B |
| Flow rate | 0.8 ml/min |
| Injection volume | 1 µl/min (1.176 pmol/ul) |
| Pressure | approx. 660 bar |
| Temperature | 45 °C |
| Detection | Fluorescence RF-20A XS (Ex 250 nm, Em 395 nm) |
| Chromatogram | 1. Asp 2. Glu 3. Ser 4. Gly 5. ? 6. Lys 7. His 8. Thr 9. Arg 10. Ala 11. AMQ 12. Pro 13. NH3 14. CysCys 15. Tyr 16. Val 17. Met 18. Ile 19. Leu 20. Lys 2 21. Phe |
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