1. Primidone (10 µg/ml); 2. Phenobarbital (10 µg/ml; 3. Phenytoin (20 µg/ml); 4. Carbamazepine (5 µg/ml)
Determination of Anticonvulsant drugs and Methyl Xanthine derivatives in serum
Article number: VPH0013J
Details
| Method | HPLC |
|---|---|
| Sample | precolumn loading and washing with Eluent A |
| Eluent | A: 14 mM NaH2PO4 - 6mM NaH2PO4 B: Eluent A / Acetonitrile / Methanol (6.5:1.5:2)
|
| Gradient | isocratic |
| Flow rate | 0.8 ml/min |
| Mode | RP Mode |
| Injection volume | 20 µl |
| Temperature | 25 °C |
| Detection | UV at 230 nm |
| Substances | Primidone, Phenobarbital, Phenytoin, Carbamazepine |
| Key words | Drugs, Anticonvulsant drugs |
| Chromatogram | 1. Primidone (10 µg/ml) 2. Phenobarbital (10 µg/ml 3. Phenytoin (20 µg/ml) 4. Carbamazepine (5 µg/ml)
|
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Chromatogram VPH0013J | 1. Primidone (10 µg/ml); 2. Phenobarbital (10 µg/ml; 3. Phenytoin (20 µg/ml); 4. Carbamazepine (5 µg/ml) |
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VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18 | 15EE181ESJ | VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.
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