1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. Thr; 6. His; 7. Ala; 8. Arg; 9. Tyr; 10. Val; 11. Cys; 12. Met; 13. Ile; 14. Phe; 15. Leu; 16. Lys
Determination of Amino acids with OPA
Article number: VPH0010J
| Method | HPLC |
|---|---|
| Sample | Amino Acid standard with OPA precolumn derivatization |
| Eluent | A: 20 mM CH3OONa in Water / Acetonitrile, (97:3) B: 20 mM CH3OONa in Water / Acetonitrile, (50:50)
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| Gradient | 5 – 28 % B, 0 – 144 s 28 – 45 % B, 145 – 560 s 45 – 82 % B, 561 – 896 s 82 – 90 % B, 897 – 1215 s
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| Flow rate | 0.6 ml/min |
| Mode | RP Mode |
| Injection volume | 1 µl |
| Temperature | 30 °C |
| Detection | Fluorescence: excitation 330 nm emission 450 nm UV at 330 nm |
| Substances | Aspartic acid, Glutamic acid, Serine, Glutamine, Histidine, Glycine, Threonine, Arginine, Alanine, Tyrosine, Methionine, Valine, Phenylalanine, Isoleucine, Leucine, Lysine |
| Key words | Amino acids, Precolumn derivatization, OPA |
| Chromatogram | 1. Asp 2. Glu 3. Ser 4. Gly 5. Thr 6. His 7. Ala 8. Arg 9. Tyr 10. Val 11. Cys 12. Met 13. Ile 14. Phe 15. Leu 16. Lys
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Chromatogram VPH0010J | 1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. Thr; 6. His; 7. Ala; 8. Arg; 9. Tyr; 10. Val; 11. Cys; 12. Met; 13. Ile; 14. Phe; 15. Leu; 16. Lys |
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VertexPlus Column 250 x 3 mm ProntoSIL 120-3 C18 H | 25CF185PSG | VertexPlus Column 250 x 3 mm ProntoSIL 120-3 C18 H |
Technical data are subject to change without notice.