1. ASP; 2. GLU; 3. ASN; 4. SER; 5. GLN; 6. HIS; 7. GLY; 8. THR; 9. ARG; 10. ALA; 11. TYR; 12. MET; 13. VAL; 14. PHE; 15. ILE; 16. LEU; 17. LYS
Determination of Amino acids in beer with precolumn derivatization (OPA)
Article number: VFD0027J
| Method | HPLC |
|---|---|
| Eluent | A: 0.05 M Acetate / Methanol (81:19) pH 7.2 B: 0.05 M Acetate / Methanol (25:75)
|
| Gradient | 0 – 28 min 100% A; 28 – 35 min 100% – 70% A; 35 – 50 min 70% – 0% A; (5 min hold), in 5 min 100% A
|
| Flow rate | 1.0 ml/min |
| Mode | RP Mode |
| Injection volume | 10 µl |
| Temperature | 40 °C |
| Detection | Fluorescence: excitation 330 nm, emission 450 nm |
| Substances | Aspartic acid, Glutamic acid, Asparagine, Serine, Glutamine, Histidine, Glycine, Threonine, Arginine, Alanine, Tyrosine, Methionine, Valine, Phenylalanine, Isoleucine, Leucine, Lysine |
| Key words | Amines Precolumn derivatization , OPA |
| Chromatogram | 1. ASP 2. GLU 3. ASN 4. SER 5. GLN 6. HIS 7. GLY 8. THR 9. ARG 10. ALA 11. TYR 12. MET 13. VAL 14. PHE 15. ILE 16. LEU 17. LYS
|
Title
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Description
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Chromatogram VFD0027J | 1. ASP; 2. GLU; 3. ASN; 4. SER; 5. GLN; 6. HIS; 7. GLY; 8. THR; 9. ARG; 10. ALA; 11. TYR; 12. MET; 13. VAL; 14. PHE; 15. ILE; 16. LEU; 17. LYS |
Technical data are subject to change without notice.