1. DNPH; 2. Formaldehyde; 3. Acetaldehyde; 4. Acrolein; 5. Propionaldehyde; 6. Acetone; 7. n-Butyraldehyde and Methacrolein; 8. Methyl ethyl ketone; 9. 2-Pentanone and Cyclohexanon
Determination of Aldehydes and Ketones as DNPH derivates
Article number: VEV0009J
| Method | HPLC |
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| Sample | Collection on a DNPH coated solid phase cartridge, elution with methanol |
| Phase | Eurospher 100-5 C18 |
| Eluent | Methanol / Water (84:16) |
| Gradient | isocratic |
| Flow rate | 0.7 ml/min |
| Mode | RP Mode |
| Injection volume | 10 µl |
| Temperature | 30 °C |
| Detection | UV at 365 nm |
| Substances | DNPH derivates of Formaldehyde, Acetaldehyde, Acrolein, Propionaldehyde, Acetone, Butyraldehyde, Methacrolein, Methyl ethyl ketone, Pentanone, Cyclohexanone |
| Key words | Aldehydes, Carbonyl compounds, Ketones, DNPH-Derivatives |
| Chromatogram | 1. DNPH 2. Formaldehyde 3. Acetaldehyde 4. Acrolein 5. Propionaldehyde 6. Acetone 7. n-Butyraldehyde and Methacrolein 8. Methyl ethyl ketone 9. 2-Pentanone and Cyclohexanon
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Chromatogram VEV0009J | 1. DNPH; 2. Formaldehyde; 3. Acetaldehyde; 4. Acrolein; 5. Propionaldehyde; 6. Acetone; 7. n-Butyraldehyde and Methacrolein; 8. Methyl ethyl ketone; 9. 2-Pentanone and Cyclohexanon |
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VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 | 25DE181ESJ | VertexPlus Column 250 x 4 mm Eurospher 100-5 C18 |
Technical data are subject to change without notice.