1. Aflatoxin G2; 2. Aflatoxin G1; 3. Aflatoxin B2; 4. Aflatoxin B1
Determination of Aflatoxins by HPLC with post column derivatization
Article number: VFD0004J
Details
| Method | HPLC |
|---|---|
| Eluent | Methanol / Water (60 / 40) / (35 / 65) |
| Gradient | isocratic |
| Flow rate | 0.7 ml/min |
| Mode | RP Mode |
| Injection volume | 20 µl / 3 µl |
| Temperature | ambient |
| Additional procedure | postcolumn derivatization with 1.3 mM bromine solution at a flow rate of 0.18 ml/min |
| Detection | fluorescence: excitation 375 nm, emission 455 nm to get a detection limit of 0.5 ppm |
| Substances | Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2 |
| Key words | Aflatoxins |
| Chromatogram | 1. Aflatoxin G2 2. Aflatoxin G1 3. Aflatoxin B2 4. Aflatoxin B1
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Chromatogram VFD0004J | 1. Aflatoxin G2; 2. Aflatoxin G1; 3. Aflatoxin B2; 4. Aflatoxin B1 |
Technical data are subject to change without notice.
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