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Group 1; 1. Delphinidol-3-glucosid; 2. Cyanidol-3-glucosid; 3. Petunidol-3-glucosid; 4. Peonidol-3-glucosid; 5. Malvidol-3-glucosid; Group 2; 6. Peonidol-3-acetylglucosid; 7. Malvidol-3-acetylglucosid; Group 3; 8. Peonidol-3-coumarylglucosid; 9. Malvidol-3-coumarylglucosid

Anthocyanins in red and rose wine

Article number: VFD0028J

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Details
Method

HPLC

Eluent

A: Water / Formic acid / Acetonitrile (87:10:3)

B: Water / Formic acid / Acetonitrile (pH 1.3)

 

Gradient

0 – 15 min 6% – 30% B

15 – 30 min 30% – 50% B

30 – 35 min 50% – 60% B

35 – 41 min 60% – 6% B

 

Flow rate

0.8 ml/min

Mode

RP Mode

Injection volume

20 µl

Temperature

40 °C

Detection

UV at 518 nm

Substances

Group 1: nonacylated anthocyanidin-3-glycosides

Group 2: acylated anthocyanidin-3-glycosides

Group 3: coumarylated anthocyanidin-3-glycosides

 

Key words

Anthocyanins

Chromatogram

Group 1

1. Delphinidol-3-glucosid

2. Cyanidol-3-glucosid

3. Petunidol-3-glucosid

4. Peonidol-3-glucosid

5. Malvidol-3-glucosid

Group 2

6. Peonidol-3-acetylglucosid

7. Malvidol-3-acetylglucosid

Group 3

8. Peonidol-3-coumarylglucosid

9. Malvidol-3-coumarylglucosid

 

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Chromatogram VFD0028J Chromatogram VFD0028J Group 1; 1. Delphinidol-3-glucosid; 2. Cyanidol-3-glucosid; 3. Petunidol-3-glucosid; 4. Peonidol-3-glucosid; 5. Malvidol-3-glucosid; Group 2; 6. Peonidol-3-acetylglucosid; 7. Malvidol-3-acetylglucosid; Group 3; 8. Peonidol-3-coumarylglucosid; 9. Malvidol-3-coumarylglucosid
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