1. Injection Peak; 2. Pamidronate; 3. Internal Standard (IS)
Analysis of Bisphosphonate Pamidronate Disodium by precolumn derivatization and fluorescence detection
Article number: VPH0024J
Details
| Method | HPLC |
|---|---|
| Eluent | A: 1 mM aqueous Na2EDTA, adjusted to pH 6.5 with 1 M NaOH B: Methanol
|
| Gradient | isocratic A:B 97:3 |
| Flow rate | 1.0 ml/min |
| Mode | RP Mode |
| Injection volume | 20 µl |
| Temperature | ambient |
| Additional procedure | a. drug-free human urine b. drug-free dog urine c. urine from a dog receiving a single intravenous injection of 5 mg of pamidronate disodium in 0.15 M saline
|
| Detection | Fluorescence: excitation 395 nm, emission 480 nm |
| Substances | Pamidronate disodium, Disodium 3-amino-1-hydroxypropylidenebisphosphonate pentahydrate (IS)
|
| Key words | Drugs |
| Chromatogram | 1. Injection Peak 2. Pamidronate 3. Internal Standard (IS)
|
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Technical data are subject to change without notice.
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